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Plasmid#86927PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and ā¦ExpressionBacterialMutationPromoterT7Available sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, followā¦ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#102951PurposeExpresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoterDepositorInsertAPEX2-NES
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTagsExpressionBacterialMutationPromoterRhamnose promoterAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYD1-mSA
Plasmid#39865DepositorInsertmonomeric streptavidin
UseTagsAga2, FLAG, and HAExpressionYeastMutationPromoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA
Plasmid#39860DepositorInsertmonomeric streptavidin
UseTags6xHis and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Traptavidin
Plasmid#24753DepositorInsertTraptavidin
UseTagsHis-6ExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G anā¦PromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Core Traptavidin
Plasmid#26054DepositorInsertCore Traptavidin
UseTagsExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G R5ā¦PromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTags6-Histidine-tagExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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rFIXX-bp chain B (IRESpuro)
Plasmid#82451PurposeChain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.DepositorInsertrFIXX-bp chain B
UseTagsBiotinExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Preā¦ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-FB-dCas9-puro
Plasmid#100547PurposeExpresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 proteinDepositorInsertsN-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin
UseCRISPRTagsFLAG, Biotin-acceptor-site (Avitag)ExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-AP-CFP-TM
Plasmid#20861DepositorInsertAP-CFP-TM
UseTagsCFP, HA, and mycExpressionMammalianMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-miniTurbo
Plasmid#209636PurposeExpression miniTurbo in mammalian cellsDepositorInsertminiTurbo
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-miniTurbo-HA
Plasmid#209637PurposeExpression miniTurbo-HA in mammalian cellsDepositorInsertminiTurbo
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mSA-EGFP-TM
Plasmid#39863DepositorInsertmonomeric streptavidin, EGFP, TM helix
UseTagsEGFP, FLAG, and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only