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  5. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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  20. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid
    #91755
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Tyr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91755-plasmid-map-sequence-id-182657

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Showing: 41 - 60 of 1600 results