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pDONR223_PRKAA1_WT
Plasmid#82274PurposeGateway Donor vector containing PRKAA1, part of the Target Accelerator Plasmid Collection.DepositorInsertPRKAA1 (PRKAA1 Human)
UseGateway entry vectorTagsExpressionMutationM10LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PRKCI
Plasmid#45263DepositorInsertPKCI (PRKCI Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_PRKRA
Plasmid#106109PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRADepositorInsertgRNA targeting PRKRA (PRKRA Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARγ
Plasmid#119915PurposeMammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARγ
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PRKCA_WT
Plasmid#82225PurposeGateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.DepositorInsertPRKCA (PRKCA Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PRKCE_WT
Plasmid#82227PurposeGateway Donor vector containing PRKCE , part of the Target Accelerator Plasmid Collection.DepositorInsertPRKCE (PRKCE Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRKCH
Plasmid#25616PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPRKCH (PRKCH Human)
UseTagsHisExpressionBacterialMutationC2 domain (amino acid residues 1-138)PromoterAvailable sinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pRK5_tAKARζ
Plasmid#119918PurposeMammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARζ
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
Plasmid#106112PurposeInducible gene expression of FlagHA-PRKRA sgRNA PAM mutantDepositorInsertPRKRA (PRKRA Human)
UseTagsFlag and HAExpressionMammalianMutationsilent mutation of Gly32 (GGG-->GGT)PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PRKCE_AFTPH
Plasmid#205901PurposeExpress mEGFP-tagged fusion protein, PRKCE_AFTPH from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK011
Plasmid#69875Purposeexpression of Channelrhodopsin-2 T159C mutantDepositorInsertChannelrhodopsin-2
UseUnspecifiedTagsExpressionMutationT159CPromoterDrosophila Hsp70Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRKCG_Halo_C_allele
Plasmid#178146PurposeDonor vector for endogenous tagging of human PRKCG at the C-terminus with halotagInsertHalotag (PRKCG Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARδ
Plasmid#119916PurposeMammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARδ
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARε
Plasmid#119917PurposeMammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARε
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5F-TACE
Plasmid#31713PurposeMammalian expression of human TACE (ADAM17)DepositorInsertTACE (ADAM17 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
SEH1L in pRK5
Plasmid#154216PurposeUntagged SEH1L cDNA expression vector for transfectionsDepositorInsertSEH1L (SEH1L Human)
UseTagsNONEExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only
