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Plasmid#54106PurposeLocalization: Intermediate Filaments, Excitation: 487, Emission: 509DepositorInsertGFAP (GFAP Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
hGFAP R239C
Plasmid#169476PurposeSingle p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAPDepositorInsertEGFP-hGFAP (GFAP Human)
UseTagsEGFPExpressionMammalianMutationChanged R 239 to CPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p_mFAP2b
Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF4n_mFAP2b
Plasmid#159464PurposeEncodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF4n_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p3_mFAP2b
Plasmid#159470PurposeEncodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p3_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-mFAP10
Plasmid#159463PurposeEncodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertmFAP10
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP2B
Plasmid#67092PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP2B (TFAP2B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRTagsExpressionMutationrs6025024, hg38:20:56638660:TTT/-PromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-iGluSnFR.A184V
Plasmid#106193PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterGFAPAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-iGluSnFR.S72A
Plasmid#106194PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72APromoterGFAPAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-hGFAP-SOX2
Plasmid#183907PurposeExpression of human SOX2 under the human GFAP ABC1D promoterDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-FAPP1
Plasmid#109555PurposeMultisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertthe PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2B-donor
Plasmid#113823PurposeCRISPR donor plasmid to tag human transcription factor TFAP2B with GFPDepositorInsertTFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGFAP R236H
Plasmid#169475PurposeSingle p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAPDepositorInsertHaloTAG mGFAP (R236H) (Gfap Mouse)
UseTagsEGFPExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mApple-GFAP-N-18
Plasmid#54906PurposeLocalization: Intermediate Filaments, Excitation: 568, Emission: 592DepositorInsertGFAP (GFAP Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gfap-Cryab
Plasmid#58947Purposehamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytesDepositorInsertCryab
UseMouse Targeting; Transgene expressionTagsExpressionMammalianMutationgenomic fragmentPromoterhuman GFAP promoter (position -2163 to +47)Available sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-KO
Plasmid#196489PurposeTo KO Gfap in mouse astrocytesDepositorInsertgRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-hArfaptin1
Plasmid#206402PurposeMammalian expression of hArfaptin1DepositorInsertArfaptin1 (ARFIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-hArfaptin2
Plasmid#206403PurposeMammalian expression of hArfaptin2DepositorInsertArfaptin2 (ARFIP2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only