Skip to main content
Addgene
Showing: 101 - 150 of 1932 results

  7. pBS cHoxD10 (CT#718)

    Plasmid
    #14010
    Depositor
    Cliff Tabin
    Insert
    HoxD10
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    14010-plasmid-map-sequence-id-182566

    Enlarge

  13. mito-PDCD10-mScarlet-I

    Plasmid
    #208887
    Purpose
    Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.
    Depositor
    Guillaume Jacquemet
    Article
    Neil J. et al BioRxiv
    Insert
    CCM3 (PDCD10 Human)
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    PDCD10 targeted to the mitonchondria
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208887-plasmid-map-sequence-id-417776

    Enlarge

  19. phSOD1-D101G-N’His

    Plasmid
    #117704
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D101G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117704-plasmid-map-sequence-id-227520

    Enlarge

  33. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid
    #139254
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV2
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139254-plasmid-map-sequence-id-325170

    Enlarge

  35. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

    Enlarge

  36. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    60600-plasmid-map-sequence-id-97110

    Enlarge

  39. pPD1025 EF1alpha TetON3G in TU6

    Plasmid
    #139262
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV6
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139262-plasmid-map-sequence-id-325375

    Enlarge

  40. pPD1026 EF1alpha TetON3G in TU2

    Plasmid
    #139258
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV2
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1alpha
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139258-plasmid-map-sequence-id-325371

    Enlarge

  46. Top7 D9T D10V N14T

    Plasmid
    #12473
    Depositor
    David Baker
    Insert
    Top7 D9T D10V N14T
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    D9T D10V N14T
    Promoter
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  47. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

    Enlarge

  48. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

    Enlarge

  49. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

    Enlarge

  50. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

    Enlarge

Showing: 101 - 150 of 1932 results