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Showing: 81 - 120 of 167 results

  1. pSBtet-GB

    Plasmid
    #60504
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60504-plasmid-map-sequence-id-97543

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  2. pSBtet-RN

    Plasmid
    #60503
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60503-plasmid-map-sequence-id-97544

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  3. pSBtet-BN

    Plasmid
    #60502
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60502-plasmid-map-sequence-id-97545

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  4. pSBtet-BB

    Plasmid
    #60505
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60505-plasmid-map-sequence-id-97542

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  5. pSBtet-GN

    Plasmid
    #60501
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60501-plasmid-map-sequence-id-97546

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  6. pSBtet-Neo

    Plasmid
    #60509
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60509-plasmid-map-sequence-id-97538

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  16. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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  19. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid
    #165976
    Purpose
    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pphlO6), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165976-plasmid-map-sequence-id-328850

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  20. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid
    #165975
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165975-plasmid-map-sequence-id-328841

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  22. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid
    #229795
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and CAG
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229795-plasmid-map-sequence-id-461775

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  24. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  25. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  28. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid
    #154881
    Purpose
    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.
    Depositor
    Hideyuki Okano
    Article
    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001
    Inserts
    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    154881-plasmid-map-sequence-id-313227

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  36. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  37. p2B-57

    Plasmid
    #177927
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET3 (contains three rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177927-plasmid-map-sequence-id-349993

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  38. pVH664

    Plasmid
    #169608
    Purpose
    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169608-plasmid-map-sequence-id-366623

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  39. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

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  40. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177925-plasmid-map-sequence-id-349995

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Showing: 81 - 120 of 167 results