Showing: 41 - 60 of 3854 results
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Plasmid#65417PurposeAAV packaging vector for hSyn driven KOR DREADD expression.DepositorUseAAVTagsHA and mCitrineExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only
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MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailabilityAcademic Institutions and Nonprofits only -
LRG (Lenti_sgRNA_EFS_GFP)
Plasmid#65656PurposeLentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter-driven sgRNA expression and EFS promo…AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-CFP;UBC-Cre25nt
Plasmid#65727PurposeExpresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.DepositorInsertsCMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression
UseCre/LoxTagsExpressionMammalianMutationPromoterCMV and UBCAvailabilityAcademic Institutions and Nonprofits only -
BPK764
Plasmid#65767PurposeBacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationPromoterT7 (x2)AvailabilityAcademic Institutions and Nonprofits only -
MSP469
Plasmid#65771PurposeHuman expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MSP2133
Plasmid#72249PurposeHuman expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationY450A, N497A, R661A, Q695A, and Q926APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MSP2443
Plasmid#72252PurposeHuman expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mCherry-T2A-Puro
Plasmid#72264PurposeExpress mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.DepositorInsertmCherry-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV
Plasmid#72265PurposeExpress gene of interest under CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pET302-6His-dCas9-Halo
Plasmid#72269PurposeEncodes a Halo-labeled fusion of nuclease deficient Cas9DepositorInsertHis6-dCas9-Halo
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCHAC-mt-mKeima
Plasmid#72342Purposeretrovirus construct for stable expressing mt-mKeimaDepositorInsertmt-mKeima
UseRetroviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mito-V5-APEX2
Plasmid#72480PurposeExpresses APEX2 in mitochondriaDepositorInsertAPEX2
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pT18mobsacB
Plasmid#72648Purposesucrose counter-selection allelic exchange vector for Pseudomonas syringaeDepositorTypeEmpty backboneUseSucrose counter-selection allelic exchange vectorTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 41 - 60 of 3854 results