Skip to main content
Addgene
Showing: 2981 - 2990 of 8673 results

  4. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

    Plasmid
    #121828
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7) (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121828-plasmid-map-sequence-id-237382

    Enlarge

  5. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid
    #121806
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121806-plasmid-map-sequence-id-237315

    Enlarge

  6. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

    Plasmid
    #121762
    Purpose
    pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    V5 epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121762-plasmid-map-sequence-id-242653

    Enlarge

  8. pMuLE ENTR U6 n.s shRNA L1-R5

    Plasmid
    #62135
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62135-plasmid-map-sequence-id-352805

    Enlarge

Showing: 2981 - 2990 of 8673 results