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pcDNA-R-iLACCO1.2

Plasmid

#208027

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPSP

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

Plasmid

#174081

Purpose

Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0

Use

Tags

NES-Flag

Expression

Mammalian

Mutation

L270G, Y271L, N311G, C313A

Promoter

CMV

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHUE411

Plasmid

#62203

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

OsU3 and Ubi

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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