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Plasmid#62175PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only
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cjBlue chromoprotein
Plasmid#117844PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, cjBlue
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT (open N-term)
Plasmid#155197PurposeEntry vector encoding non-tagged O-GlcNAc Transferase with open N-terminusDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway cloningTagsExpressionMutationWT OGT with no start codon, in-frame with gateway…PromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
meffRed chromoprotein
Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR E2 HSPter-35Sp-mito
Plasmid#158727PurposePlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4DepositorInsertA terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
Plasmid#136382PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-VPR
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Pink chromoprotein
Plasmid#117851PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Pink
UseSynthetic Biology; Escherichia coliTagsExpressionMutation64A,65C, BioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆UBR
Plasmid#81063PurposeGateway entry vector encoding full length UBR5 W1235L (∆UBR)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
UseCloningTagsExpressionMutationW1235L (bp g3704t)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-EGFP-sv40
Plasmid#184411PurposeAAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-EGFP (Htr5b Mouse)
UseAAVTagsEGFPExpressionMammalianMutationPromoterMouse Htr5b geneAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…ExpressionMutationPromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_3xflag_NRF2
Plasmid#136527PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A3
Plasmid#132160PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A3 (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2T-CAG-SpCas9-BlastR
Plasmid#107190PurposeConfers constitutive expression of SpCas9DepositorInsertSpCas9 (NEWENTRY )
UseCRISPRTags3xFLAGExpressionBacterial and MammalianMutationPromoterChicken ß-actinAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-VPS35
Plasmid#21689DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseGateway entry cloneTagsExpressionMutationlast nucleotide of stop codon removed for c-termi…PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTarget TMEM97-P2A-mCardinal
Plasmid#157747PurposeExpress in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.DepositorInsertTMEM97 (TMEM97 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only