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Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-V660N
Plasmid#12292DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y and V660N; signal peptide (aa1-23) re…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
C513-E07: attB5r-<p10p-polyhedrinp>-attB1r
Plasmid#162949PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG NUFIP1 W40A
Plasmid#110951Purposeexpresses mammalian FLAG-NUFIP1 W40ADepositorInsertNUFIP1 W40A (NUFIP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged Tryptophan 40 to alaninePromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-P681H
Plasmid#12290DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationA74V, M82I, H202Y and P681H; signal peptide (aa1-…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG418R-GMRWhite
Plasmid#165883PurposeG418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW2
Plasmid#166708Purposeexpresses NEDD4L with inactivating mutation in second WW domain in mammalian cellsDepositorInsertNEDD4L W453A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 453 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW3
Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-1st WW mutant
Plasmid#18998DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 199 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ255
Plasmid#124310PurposeGateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editingDepositorInsertrAPOBEC1-pcoCas9D10A-UGI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_Plk5(WT)
Plasmid#136329PurposeHuman Plk5 WT in Gateway pENTRDepositorInsertPLK5 (PLK5 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3x-Flag Gli3 37R3-2
Plasmid#120305PurposeExpresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37CDepositorInsertIntein 37R3-2, Gli3
UseSynthetic BiologyTags3X FlagExpressionMammalianMutationContains mutations as reported in 2011 Chemistry …PromoterCMVAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-2nd WW mutant
Plasmid#18999DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutationTryptophan 258 changed to Alanine, and Proline 2…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_EWSR1_His6
Plasmid#26364DepositorInsertEWSR1 (EWSR1 Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 657 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only