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ancBE4-NG

Plasmid

#226853

Purpose

Expresses ancBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ancBE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ancBE4max-SpRY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mCitrine-N1

Plasmid

#54594

Purpose

Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Roger Tsien, Oliver Griesbeck, Robert Campbell, Michael Davidson

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-dCas13-M3nls

Plasmid

#155366

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-p18-FLAG

Plasmid

#42331

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pCMV_BE4max_3xHA

Plasmid

#112096

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pIS2

Plasmid

#12177

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMVTRE3G eGFP Blast (w818-1)

Plasmid

#27568

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 236HSC WT (BRCA2)

Plasmid

#16246

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-CD151-7

Plasmid

#57125

Purpose

Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

CD151 (CD151 Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-LUC2CP/ARE

Plasmid

#62857

Purpose

Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

destabilizing sequences (DS) added to the C termi…

Promoter

CMV

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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AICSDP-47: CTNNB1-mEGFP

Plasmid

#109119

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

Depositor

Allen Institute for Cell Science

Insert

CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

May 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2-C1

Plasmid

#54550

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Tao Xu, Michael Davidson

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET23a_mod_HuD_FL

Plasmid

#65751

Purpose

plasmid for bacterial overexpression of HuD

Depositor

Thomas Tuschl

Insert

ELAVL4 (ELAVL4 Human)

Use

Tags

6X His and FLAG HA

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

Plasmid

#183776

Purpose

AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

Cre

Use

AAV

Tags

Expression

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB modified

Plasmid

#22226

Depositor

David Baltimore

Article

Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge