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lenti SYN-dCas9-KRAB-MeCP2
Plasmid#155365PurposeExpresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoterDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTags3x FLAG and 7x HisExpressionMammalianMutationPromoterSYNAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantMutationPromoter2x35SAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-SINTBAD
Plasmid#210209PurposeFor mammalian expression of SINTBAD (Internal ref. SMC2249)DepositorInsertTBKBP1 (TBKBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-44B12mut23(Ser5ph)-SNAP
Plasmid#174877PurposePiggyBac transposon vector for expression of RNAPII Ser5ph mintbodyDepositorInsertRNAPII Ser5ph mintbody-SNAP
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 gamma
Plasmid#24076DepositorInsertHP1 gamma (CBX3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.CaMPARI.WPRE.SV40
Plasmid#100832PurposeAAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integratorDepositorHas ServiceAAV1, AAV5, and AAV9InsertCaMPARI
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
UseTagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a
Plasmid#80611PurposepBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Syndapin1fl
Plasmid#36859DepositorInsertSyndapin1 (Pacsin1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Claudin-1
Plasmid#46387PurposeClaudin-1 promoter reporter assayDepositorInsertCLDN1 Promoter (CLDN1 Human)
UseLuciferaseTagsExpressionMutationPromoterCLDN1 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR2 (CXCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:EGFP
Plasmid#127288Purposeall-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)DepositorInsertNgn2:2A:Ascl1 (NEUROG2 Human)
UseLentiviralTagsNgn2 and EGFP linked via 2AExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Syntaxin1A
Plasmid#170871PurposeMammalian expression of His-GFP-Syntaxin1A.DepositorInsertSyntaxin 1A (Stx1a Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyto-Ruby3-iATPSnFR1.0
Plasmid#102551Purposeexpresses cytoplasmic ATP sensor in mammalian cells with red reference proteinDepositorInsertRuby3-iATPSnFR1.0
UseTagsRuby3ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z07
Plasmid#124790PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only
