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Plasmid#107730PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1
Plasmid#107729PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-pEF-EGFP
Plasmid#67952PurposeLentiviral vector for cloning in miRNA target sequences following EGFP sequenceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a C2AB
Plasmid#40051DepositorInserthSlp2-a C2AB (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationC2AB domains only (aa 597-910)PromoterCMV promoterAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFYF1328 EGFP Site#3
Plasmid#47513Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
B108-GFP-IRES-AP
Plasmid#73988PurposeB108 enhancer of Blimp1 gene drives EGFP expression.DepositorInsertB108 enhancer
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IC2-N106
Plasmid#51411Purposeexpression of GFP-tagged N-terminal AA 1-106 of dynein intermediate chain IC2C in mammalian cellsDepositorInsertdynein intermediate chain IC2C (Dync1i2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP-ccdB
Plasmid#14315DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoNanobody IRES EGFP
Plasmid#136621PurposePlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pnGFP-Ultra
Plasmid#157923PurposePeroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)DepositorInsertpnGFP-Ultra, a high performance peroxynitrite sensor
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-VASP-5
Plasmid#57161PurposeLocalization: Actin/Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertVASP
UseTagsmPA-GFPExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-hSPC-TFEBeGFP-W
Plasmid#126692PurposeLineage specific over expression of TFEB in lung alveolar epithelial cellsDepositorInsertTFEB (TFEB Human)
UseLentiviralTagsExpressionMutationDeleted Stop Codon on TFEBPromoterhSPCAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-alpha2-chimaerin
Plasmid#59316PurposeExpression plasmid for GFP-tagged alpha2-chimaerin (GFP at N-terminus of alpha2-chimaerin)DepositorInsertalpha2-chimaerin
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-GFP
Plasmid#120903PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(R111G)
Plasmid#110187PurposeExpression of mouse MeCP2 (R111G) in mammalian cellsDepositorInsertMeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationArg 111 GlyPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v3-sfGFP
Plasmid#145174PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 3DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EGFP
Plasmid#14190DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF138-delta 18-58
Plasmid#78921PurposeMammalian expression of RNF138 with delta 18-58 and an EGFP fusionDepositorInsertRNF138 (RNF138 Human)
UseTagsEGFPExpressionMammalianMutationdelta 18-58PromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only