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Showing: 5881 - 5900 of 34824 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129031
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA3 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129031-plasmid-map-sequence-id-256155

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  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129032-plasmid-map-sequence-id-256156

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  4. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107578-plasmid-map-sequence-id-209151

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  5. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  6. pdCas9-M-4F2

    Plasmid
    #73431
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4F2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73431-plasmid-map-sequence-id-135331

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  7. pdCas9-M-1B6

    Plasmid
    #73430
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1B6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73430-plasmid-map-sequence-id-139104

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  8. pdCas9-M-3H5

    Plasmid
    #73429
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3H5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73429-plasmid-map-sequence-id-135329

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  9. pdCas9-M-1D4

    Plasmid
    #73433
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1D4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73433-plasmid-map-sequence-id-135333

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  10. pdCas9-M-1E4

    Plasmid
    #73436
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1E4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73436-plasmid-map-sequence-id-135335

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  11. pdCas9-M-5F5

    Plasmid
    #73432
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 5F5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73432-plasmid-map-sequence-id-135332

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  14. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  15. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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  16. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107582
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fba promoter Bacillus megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107582-plasmid-map-sequence-id-209150

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Showing: 5881 - 5900 of 34824 results