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Plasmid#210364PurposeExpresses REEP6 fused with EGFP in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA15-Ubc-NLS- scFV-tdTomato
Plasmid#199446PurposeExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cellsDepositorInsertscFV-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform7
Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorInserthsOPA1 isoform 7 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.pU1a-SpCas9
Plasmid#121507PurposeExpresses codon-optimized SpCas9 in mammalian cells.DepositorInsertSpCas9
UseTagsHAExpressionMammalianMutationPromoterU1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
UseTagsHis-tag and Mitochondria localization sequenceExpressionMammalianMutationPromoterCMV, SP6Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx
Plasmid#42596DepositorInsertHepatitis B virus protein X
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-FLAG-NES
Plasmid#188525PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-pDUXCca
Plasmid#198236PurposeLentivector with tet-inducible porcine DUXC (codon altered)DepositorInsertporcine DUXC (codon altered)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF149 pSOD1G85RAcGFP1
Plasmid#26410DepositorInsertSOD1 (SOD1 Human)
UseTagsGFPExpressionMammalianMutationG85RPromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5A-CA-CMV-GFP
Plasmid#83255PurposeAdenoviral expression of constitutively active STAT5A with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationconstitutively activePromoterCMVAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-YFP
Plasmid#15302DepositorInsertYFP
UseGateway entryTagsExpressionMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
sIL6R PLAK1
Plasmid#161519PurposeExpresses soluble IL6 receptor in mammalian cells.DepositorInsertsoluble IL-6 receptor
UseLentiviralTags3X FlagExpressionMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3
Plasmid#119972PurposeLentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En01
Plasmid#71534PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
FL tension sensor module
Plasmid#101170PurposeExpresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.DepositorInsertYPet(short)-FL-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 mBAX
Plasmid#129577PurposeCRISPR deletion of murine BAXDepositorInsertgRNA vs murine BAX (Bax Mouse)
UseLentiviralTagsExpressionMammalianMutationNonePromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
UseTagsExpressionBacterialMutationPromoterglnS' and lambda(ts) and proKAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only