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[GFP][IRES][Bla]

Plasmid

#97194

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-L269P-EGFP

Plasmid

#132601

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

806T>C, 807G>C; Leu269Pro

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P441L-EGFP

Plasmid

#132604

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1322C>T, 1323A>G; Pro441Leu

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-AlstR

Plasmid

#32647

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
AlstR (AstA-R1 Fly)

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
Phage-PGK-GFP-T(WT)

Plasmid

#181736

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Insert

T (brachyury)

Use

Lentiviral

Tags

Expression

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-fSFRP2-GFP

Plasmid

#22915

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu secreted frizzled receptor protein 2 promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-N3-FLuc-K0-Rluc_AH

Plasmid

#148846

Purpose

Mammalian Expression of K0-Rluc

Depositor

Elisa Izaurralde

Insert

K0-Rluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFP

Plasmid

#166676

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-PrCas12a-2AeGFP

Plasmid

#123636

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PrCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJMD-In3.4:EGFP

Plasmid

#44662

Depositor

John Rawls

Inserts

Angptl4 Intronic Region 3.4
Minimal cFOS
EGFP

Use

Tol2 vector

Tags

Expression

Mutation

Promoter

cFOS

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-Xl-VSP1-GFP

Plasmid

#51883

Purpose

expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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ATF4 12: 5’ATF4.uORF1AUA.GFP

Plasmid

#21859

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF1 was converted to AUA

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP

Plasmid

#182737

Purpose

GFP expression under the control of CamKII promotor

Depositor

Janelia Viral Tools

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

CamKII

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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H2A-Bbd-GFP

Plasmid

#45173

Depositor

Hunt Willard, Brian Chadwick

Article

Chadwick et al J Cell Biol. 2001 Jan 22;152(2):375-84.

Insert

H2AFB1 (H2AFB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-ARAP3(R307,8A)

Plasmid

#39486

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3(R307,8A) (ARAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R307,8A = PH domain mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAAVsc.U6-sgFah.CMV/CB-EGFP

Plasmid

#121508

Purpose

Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 S156E

Plasmid

#127589

Purpose

Phospho-mimetic of S156

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Miro1 (RHOT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S156E

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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