Addgene

We narrowed to 8,441 results for: CAG

Showing: 5861 - 5880 of 8441 results

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pLenti CRISPR V2 sgMTHFD1L_1

Plasmid

#106316

Purpose

Express Cas9 and sgRNA targeting MTHFD1L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD1L

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TDP43 NOTAG2

Plasmid

#28207

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

aa86-414 only

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Use

Tags

Expression

Mutation

Promoter

T7

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

Plasmid

#42046

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

Plasmid

#42045

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Promoter

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX58-ts1

Plasmid

#174241

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-KLF7-1

Plasmid

#169797

Purpose

Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

guide RNA for KLF7 neighbouring region

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Worm and Yeast

Mutation

Promoter

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1333

Plasmid

#119268

Purpose

rfp "test" strain

Depositor

Jason Peters, Oren Rosenberg, Carol Gross

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-FOXP2

Plasmid

#47053

Purpose

Expresses forkhead box P2

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Forkhead Box P2 (FOXP2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

Plasmid

#182344

Purpose

sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Spacer 4

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FRB-GEM23-Halo

Plasmid

#197060

Purpose

Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

I3-01

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-SETin-FLAG-HA

Plasmid

#24999

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations introduced to prevent siRNA targ…

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
MBP-TSF-TEVcs-ULK1

Plasmid

#171416

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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