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Plasmid#203740PurposeIntegration of OsTIR1-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationPromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP700
Plasmid#203742PurposeC-terminal tagging of target proteins with AID*/3xHA degronDepositorInsertIAA17 auxin binding domain codons 71-114
UsePcr amplification template for gene taggingTags3xHAExpressionMutationPromoternoneAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP701
Plasmid#203743PurposeC-terminal tagging of target proteins with 3xV5-IAA17 degronDepositorInsertIAA17 auxin binding domain
UsePcr amplification template for gene taggingTags3xV5ExpressionMutationPromoternoneAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…PromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial cpGFP
Plasmid#215711PurposeExpresses cpGFP in the mitochondriaDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T1
Plasmid#80495PurposeAAVS1 TALEN expression vector (Left)DepositorInsertAAVS1 TALEN (Left)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T2
Plasmid#80496PurposeAAVS1 TALEN expression vector (Right)DepositorInsertAAVS1 TALEN (Right)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEGFP
Plasmid#18696DepositorInsertmonomeric EGFP
UseTagsExpressionMammalianMutationA206K mutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6-tmpknot-GG-acceptor
Plasmid#174039PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_52
Plasmid#109250PurposeColE1 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_42
Plasmid#109240PurposepSC101 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo largeTcDNA
Plasmid#1780PurposeRetroviral expression of Large T Antigen. Used to create immortalized cell lines.DepositorInsertlarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
GFP-SMARCA4
Plasmid#65391PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE GFP
Plasmid#10668PurposeEmpty backbone for retroviral gene expression. Select recipient cells using GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSPL
Plasmid#110743PurposePlasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)
Plasmid#26736DepositorInsertBACE (Bace1 Mouse)
UseTagsFLAGExpressionMammalianMutationCys 474, 478, 482, and 485 mutated to AlaPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA
Plasmid#53272PurposeContains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertcrtE, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-lux
Plasmid#64963Purposemini-Tn7 luxCDABE transcriptional fusion vectorDepositorInsertlux operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits