We narrowed to 15,329 results for: grna

1. pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128343

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrnb2 (Chrnb2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

   Plasmid

   #159660

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

   Plasmid

   #159661

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

   Plasmid

   #159659

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128340

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna5 (Chrna5 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197024

   Purpose

   Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized OsCas12f1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

   Plasmid

   #160621

   Purpose

   Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26:gRNA 4 (pNos): 2.1 aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

   Plasmid

   #136270

   Purpose

   Expresses ABE and ST2-com modified sgRNA scaffold

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Insert

   ABE and sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109311

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Insert

   Ldlr gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid

    #113692

    Purpose

    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

    Plasmid

    #109317

    Purpose

    Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121816

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197025

    Purpose

    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized RhCas12f1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge