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pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126778

Purpose

Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R691A

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

Plasmid

#121813

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Olig2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121819

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126777

Purpose

Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

F539S, M763I, K890N

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124288

Purpose

sgRNA against human NFATc3

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc3 nuclear factor of activated T cells (NFATC3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124286

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

Plasmid

#228362

Purpose

mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

Depositor

Michael Boettcher

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Tags

Expression

Mammalian

Mutation

Promoter

mU6 and hU6

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

Plasmid

#220129

Purpose

Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSauri Cas9C

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

Plasmid

#223162

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223161

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

Plasmid

#206860

Purpose

An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

Depositor

William Lagor

Article

De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

Insert

mLdlr gRNA (Ldlr Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

Plasmid

#131683

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

EF1a and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA

Plasmid

#209788

Purpose

Expresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

SpRY C, Camk2d sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

CASI

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

Plasmid

#208110

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, Lmna sgRNA1, scaffold

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCF827-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211693

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-1 guide RNA vector

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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