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Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_015
Plasmid#123991PurposeEncodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthROSA26 sg4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8_005
Plasmid#123885PurposeEncodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK systemDepositorInsertF1-KanR-ColE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_021
Plasmid#123936PurposeEncodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK systemDepositorInsertTUL3R4 spacer
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS160
Plasmid#134238PurposeLentiviral plasmid expressing sgRNA against HJURPDepositorInsertHuman HJURP sgRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM328
Plasmid#134243PurposeMammalian expression construct expressing sgRNA against PLK1DepositorInsertgRNA targeting PLK1 3'
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-pT7-sfGFP_RED20
Plasmid#135173PurposeExpresses sfGFP recoded in the RED20 genetic code off the T7 promoterDepositorInsertsfGFP_RED20
UseSynthetic BiologyTagsExpressionMutationRecoded ORF of sfGFP to only use one codon per am…PromotertaatacgactcactataAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_047
Plasmid#124055PurposeEncodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_048
Plasmid#124056PurposeEncodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_049
Plasmid#124057PurposeEncodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB340
Plasmid#124023PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713DepositorInsertLS-A4_EF1a-NLS::iRFP713-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB341
Plasmid#124024PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFPDepositorInsertLS-A4_EF1a-NLS::tagBFP-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB344
Plasmid#124027PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713DepositorInsertL1-B1_CMV-NES::iRFP713-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB345
Plasmid#124028PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFPDepositorInsertL1-B1_CMV-NES::tagBFP-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB346
Plasmid#124029PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreenDepositorInsertL1-B1_CMV-NES::mAzamiGreen-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB347
Plasmid#124030PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2DepositorInsertL1-B1_CMV-NES::mRuby2-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB348
Plasmid#124031PurposeEncodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged iRFP713DepositorInsertL2-C3_CAG-Lyn::iRFP713-SV40_RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only