Addgene

Showing: 561 - 580 of 781 results

Results per page:

Sort results by:

p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

Plasmid

#216475

Purpose

lentiviral dual expression of YAP5SA and eGFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
eGFP

Use

Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_Puro_Telo_v2

Plasmid

#195139

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

eGFP and iLID

Expression

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_Puro_Telo_v1

Plasmid

#195138

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

modified bovine U6-2

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Neo_POU2F3_CR

Plasmid

#122175

Purpose

Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

3 silent mutations for gRNA resistance (882g-c, 8…

Promoter

EFS

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Neo_SIK3_CR_T221E

Plasmid

#108108

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation),…

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-M2-CDC27 wt

Plasmid

#31661

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog (CDC27 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
R713-M91-303: CMV51p> Halotag-Hs.NRAS

Plasmid

#159682

Purpose

Mammalian protein expression of NRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.NRAS (NRAS Human)

Use

Tags

Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PPP1R12A wt

Plasmid

#31669

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CRAF_S259A_pcDNA3.1

Plasmid

#86505

Purpose

Expresses CRAF_S259A in mammalian cells

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S259A

Promoter

CMV

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Rab8-T22N(Flag) in pcDNA3.1neo

Plasmid

#46784

Purpose

expression of T22N Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A T22N (RAB8A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T22N dominant negative

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-CDC27 wt

Plasmid

#31673

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog (CDC27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-AUP1-FLAG 5E

Plasmid

#185341

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

R382E/Q383E/K390E/R398E/R400E

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge