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pPGK-T7/2-CD44v8-10 (fl)

Plasmid

#137823

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6-10 (-cyt)

Plasmid

#137821

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v8-10 (-cyt)

Plasmid

#137822

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v2-10 (fl)

Plasmid

#137824

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length and mutations A282T, T483A, N535D, S5…

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pαH-S-GSAS-OMICRON.BA.2-RBD

Plasmid

#184830

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP-G3BP1(2)

Plasmid

#136059

Purpose

G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEW3: pET-32a Dot1L(2-416)

Plasmid

#124098

Purpose

Dot1L(2-416) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-416) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Use

Tags

Trx-His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-GFP-G3BP2(2)

Plasmid

#136061

Purpose

G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
LB595 (Bcl-2 promoter CRE mut)

Plasmid

#15243

Depositor

Linda Boxer

Article

Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Mutated CRE site at -1537 changed from GTGACGTTA …

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB322 (Bcl-2 from ATG to -3934)

Plasmid

#15381

Depositor

Linda Boxer

Article

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -3934 with both P1 and…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1376 (Bcl-2 P1 promoter with MHS1234)

Plasmid

#15387

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P1 promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
LB335 (Bcl-2 from ATG to -1281)

Plasmid

#15382

Depositor

Linda Boxer

Article

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -1281 with P2

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1377 (Bcl-2 P2 promoter with MHS1234)

Plasmid

#15386

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P2 promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only