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Addgene
Showing: 561 - 573 of 573 results
  1. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pUC19-J9E534

    Plasmid
    #103078
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Insert
    J9E534(Cas9 coding gene from Alicyclobacillus hesperidum URH17-3-68)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pUC19-C7TEQ6

    Plasmid
    #103092
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Insert
    C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pIALD2HMGr

    Plasmid
    #185867
    Purpose
    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae
    Depositor
    Insert
    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pMXS-mitoGshF-blasticidin

    Plasmid
    #210314
    Purpose
    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…
    Expression
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 561 - 573 of 573 results