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FLAG-KIF17-short-pcDNA3
Plasmid#47545PurposeExpresses FLAG epitope-tagged short form of mouse KIF17 in mammalian cellsDepositorInsertKIF17-short (Kif17 Mouse)
UseTagsFLAG epitopeExpressionMammalianMutationG152S*PromoterCMVAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lck-GFP
Plasmid#61099PurposeExpression of a membrane-targeted form of eGFPDepositorInsertLck-GFP
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe:GFP
Plasmid#13788PurposeMammalian expression of enhanced form of Flp recombinase fused to GFPDepositorInsertFlpe-GFP fusion protein
UseFlp/frtTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro H-Ras V12
Plasmid#9051PurposeRetroviral expression of a constitutively active form of H-RasDepositorInsertHras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET-NDH6
Plasmid#105503PurposeExpression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tagDepositorInsertNDH6
UseTags6xHisExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p307HU
Plasmid#27386DepositorInsertunligated form of ligase ribozyme
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pSP64T3 BMP2 pro Vg1 (DM#119)
Plasmid#15071DepositorInsertBMP2-Vg1
UseSp6 expression for use in xenopusTagsExpressionMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOET5 CVB3-VLP
Plasmid#218146PurposeForms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).DepositorInsertsVP1–VP4
3CD
UseTagsExpressionInsectMutationPromoterCMV and PolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 GII.4 Sydney noro-VLP
Plasmid#218092PurposeForms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).DepositorInsertnoro-VLP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ARL16-His6
Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP NOVA2 op
Plasmid#224354PurposeExpress GFP-tagged human NOVA2 (codon optimized)DepositorInsertNOVA2 (NOVA2 Human)
UseTagseGFPExpressionMammalianMutationcodon optimized for expression in human cellsPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)
Plasmid#194175PurposeExpresses lncRNA HIF1a-AS1 mutantDepositorInsertHIF1a-AS1 (HIF1A-AS1 Human)
UseTagsExpressionMammalianMutationWithin Exon1, nucleotides 26-78 were removedPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta55-118-10xHis
Plasmid#194180PurposeExpresses MPP8-delta55-118_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta55-118 (chromo domain deletion)PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2CreERT2
Plasmid#13777PurposeMammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-Rab9aS21N
Plasmid#169073PurposeExpress human dominant negative form of Rab9a(S21N) in mammalian cellsDepositorInsertRab9a (RAB9A Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry
Plasmid#214470PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F
Plasmid#39264DepositorTypeEmpty backboneUseBacterial expression, bacterial expression of re…Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA-ISceID44A
Plasmid#59424PurposeExpresses a catalytically inactive form of the endonuclease ISceIDepositorInsertendonuclease ISceI mutant (I-SceI )
UseTagsHA tagExpressionMammalianMutationchanged aspartate 44 to alanine (D44A)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG5 E107K T Ag
Plasmid#133710PurposeLarge T antigen mutant form in pSG5 vector for mammalian expression.DepositorInsertSV40gp6 (SV40gp6 SV40)
UseTagsExpressionMammalianMutationE107KPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tm2AG
Plasmid#26773DepositorInsertTdTomato-2A-H2BGFP-SV40pA
UseTagsExpressionMammalianMutationmutation in 2A peptide that renders it non-functi…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO3
Plasmid#53143PurposeE.coli expression of untagged mature human SUMO3 (amino acids 1-93)DepositorInsertSUMO3 (SUMO2 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-93)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO2
Plasmid#53142PurposeE.coli expression of untagged mature human SUMO2 (amino acids 1-92)DepositorInsertSUMO2 (SUMO3 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-92)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro K-Ras V12
Plasmid#9052PurposeRetroviral expression of a constitutively active form of K-RasDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V. Constitutively active.PromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promEco/Lysostaphin
Plasmid#125765PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK-Sa1 promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald
Plasmid#201186PurposeLentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RanWT-mRFP1-polyA
Plasmid#59750PurposeExpress wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan-mRFP1
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDsEndoNA2
Plasmid#174728PurposeExpresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertDsRed-Monomer
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CD44Z-c011
Plasmid#175455PurposeProtein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-kRasV12
Plasmid#114318PurposeTET-inducible expression of the V12-mutant form of the ki-Ras proteinDepositorInsertRas (KRAS Human)
UseLentiviralTagsExpressionMutationG12V oncogenic mutation. Constitutively active K-…PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab9aQ66L
Plasmid#169072PurposeExpress human constitutively active form of Rab9a(Q66L) in mammalian cellsDepositorInsertRab9a (RAB9A Human)
UseTagsacGFP1ExpressionMammalianMutationQ66LPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro
Plasmid#177201Purposeencoding a dominant negative form of CEBP under inducible promoterDepositorArticleInsertdnCEBP (A-C/EBP)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
RISAP act beta-catenin (CT#1147)
Plasmid#14041DepositorInsertbeta catenin (ctnnb1.L Frog)
UseRetroviral; Avian expressionTagsExpressionMutationActivated formPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
V5-W279A-short-UBASH3A
Plasmid#192086PurposeMammalian expression of human W279A short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationW279APromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mERRbeta-short
Plasmid#52188PurposeExpresses mouse ERRbeta short form splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only
