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Showing: 1121 - 1160 of 23151 results

  4. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  5. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  11. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  12. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  15. pYZ673

    Plasmid
    #132953
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    FnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    adh1
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    132953-plasmid-map-sequence-id-310101

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  26. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Quanjiang Ji
    Article
    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    117235-plasmid-map-sequence-id-227412

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  30. pCP-tRNA

    Plasmid
    #133812
    Purpose
    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
    Depositor
    Geraldine Butler
    Article
    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001
    Insert
    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133812-plasmid-map-sequence-id-263618

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  31. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  33. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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  34. pYZ033

    Plasmid
    #98404
    Purpose
    Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98404-plasmid-map-sequence-id-198860

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  37. pAH237

    Plasmid
    #121146
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Mutation
    Promoter
    S.pombe rrk1 and nmt41
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121146-plasmid-map-sequence-id-234796

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Showing: 1121 - 1160 of 23151 results