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Plasmid#39977DepositorInsertTrkA (Ntrk1 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-PLCdelta-PH
Plasmid#71375PurposeFor analysis of PH domain localization in yeastDepositorInsertPLCdelta-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domainPromoterAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-CAMKIIbeta(T287D)
Plasmid#21223DepositorInsertCAMKIIbeta (Camk2b Rat)
UseTagsGFPExpressionMammalianMutationCAMKIIbeta(T287D) Lab plasmid CF 0001.PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-IRES2-p75NTR-GFP
Plasmid#182947PurposeTo express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-TerminalDepositorInsertp75NTR-mGFP
UseTagsHA tag and mGFPExpressionMammalianMutationThe leader sequence is from preprotrypinPromoterCAGAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
Plasmid#104496PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Lysosomes-20
Plasmid#55428PurposeLocalization: Lysosome Membrane, Excitation: 433, Emission: 475DepositorInsertLAMP1
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Halo-p58
Plasmid#166896Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTagDepositorInsertp58 (Lman1 Rat)
UseTagsHaloTagExpressionMammalianMutationL405Q, R419S- please see depositor commentsPromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-HaloTag
Plasmid#66993PurposeG protein alpha-s internally tagged with HaloTag and EE epitopeDepositorInsertG-alpha-s-EE-HaloTag (Gnas Rat)
UseTagsHaloTag flanked by SGGGGSGGGGS on each side was i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7b-WPRE
Plasmid#104489PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjGCaMP7b
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cx43-7
Plasmid#55023PurposeLocalization: Gap Junctions, Excitation: 587, Emission: 610DepositorInsertCx43 (Gja1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
NGL-1-myc/pEGFP-N1
Plasmid#197314PurposePlasmid expressing an antigen targeting Neuroligin-1DepositorInsertNeuroligin-1 (Nlgn1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-irisin-his
Plasmid#122729PurposeExpresses in mammalian cells (HEK293):glycosylated and secreted irisin-hisDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Bovine, Mouse, Human, Rat)
UseTagshis and signal sequence for secretionExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rat Alpha2 delta-1 HA pMT2
Plasmid#58729Purposeexpression of HA tagged rat Alpha2 delta-1 in mamalian cellsDepositorInsertalpha2 delta-1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Lysosomes-20
Plasmid#57793PurposeLocalization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLAMP1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD50E in LAMP1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
JWW-1 human chimeric antibody
Plasmid#66748PurposeExpresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.DepositorInsertsJWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa
UseTagsExpressionMammalianMutationFirst 3 amino acids of rat light chain constant r…PromotermEF1 and rEF1Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPA016 (delta 86-130 Parkin)
Plasmid#102357Purposeexpresses Rat Parkin with residues 86-130 deletedDepositorInsertParkin (Prkn Rat)
UseTagsGSTExpressionBacterialMutationdeleted residues 86 to 130PromotertacAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)
Plasmid#165440PurposeExpresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)
UseTagsmEGFP(A206K)ExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFlag-CMV7-Erk2
Plasmid#39223DepositorInsertErk2 (Mapk1 Rat)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pl-Synapsin-YFP-NaVII-III
Plasmid#91246PurposeExpresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)DepositorInsertNav1.2 (Scn2a Rat)
UseLentiviralTagsYFPExpressionMammalianMutationThe insert codes only for the intracellular loop …PromoterAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only