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pRRL-5xTetO-pEF-H2B-Citrine-SV40

Plasmid

#186966

Purpose

Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

5xTetO-pEF-H2B-Citrine-SV40

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

5xTetO-pEF-1alpha

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-BICD1

Plasmid

#49487

Purpose

expresses EGFP-BICD1 in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu

Insert

BICD1 (BICD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

** see notes

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP

Plasmid

#111518

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56AD93A/D129A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D93A/D129A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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CaM12(D20A/D56A)/pIRES2-eGFP

Plasmid

#111512

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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ME2/pBABE-puro

Plasmid

#49164

Purpose

For expression of Malic Enzyme 2 in mammalian cells from retroviral vector

Depositor

Xiaolu Yang

Article

Jiang et al Nature. 2013 Jan 31;493(7434):689-93. do

Insert

Malic Enzyme 2 (ME2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Article

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Entry vector for gateway cloning

Tags

mCherry superfolder GFP

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

Plasmid

#200123

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TdIF2

Plasmid

#199699

Purpose

visualization of nucleolus in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

TdIF2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-bphD

Plasmid

#128138

Purpose

The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Use

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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Daxx/pEGFP

Plasmid

#119021

Purpose

Express GFP-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

death-domain associated protein (Daxx) (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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YPET-GGA3

Plasmid

#18841

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

YPET-GGA3 (GGA3 Human)

Use

Tags

His6 and myc

Expression

Mammalian

Mutation

The fluorescent protein YPET was added to the ami…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
pNPK-NLS-Vika

Plasmid

#79969

Purpose

expresses human codon-optimized Vika-recombinase in mammalian cells

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

human codon-optimized Vika sequence, NLS tagged

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_UGControl

Plasmid

#133875

Purpose

Expresses UGControl in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Use

Tags

flexible linker - mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_UG3

Plasmid

#133874

Purpose

Expresses UG3 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Use

Tags

helical linker - mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-SnFR-gamma2-minWPRE

Plasmid

#165497

Purpose

AAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisation

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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