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pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shPrc1

Plasmid

#160959

Purpose

Prc1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Prc1 shRNA (Prc1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1

Plasmid

#22420

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Use

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #2

Plasmid

#83090

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pSL1180 EACMCV DNA-B 1.6mer

Plasmid

#159137

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-B

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-B 1.5mer

Use

Infectious clone for bombarding plants

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript II KS(+) EACMCV DNA-A 1.6mer

Plasmid

#159136

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

EACMCV DNA-A 1.6mer

Use

Infectious clone for bombarding plants

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S208A

Plasmid

#27115

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

S208A

Promoter

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Use

Tags

Expression

Bacterial

Mutation

109

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p42)

Plasmid

#135941

Purpose

Expresses AUF1p42 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP T184M

Plasmid

#114181

Purpose

ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP T184M (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

T184M

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 AT1AR-324delta

Plasmid

#45635

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-324delta (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

added stop codon at 324 aa

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 delta 610-615

Plasmid

#19941

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta 610-615 (CUL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CUL1 with a ROC1 binding mutant

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
3xFN-Tel-TAL/pMXs-puro

Plasmid

#63590

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

Retroviral and TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA INPP5E KD1

Plasmid

#162004

Purpose

INPP5E shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5E (INPP5E Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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