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PB-CT3G-CERP2
Plasmid#175499PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
NylCK-TS_pET28a
Plasmid#215423PurposeExpression construct for NylCK-TS gene, codon optimized for expression in E. coliDepositorInsertNylCK-TS
UseTagsExpressionBacterialMutationS111G/A137LPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA007
Plasmid#131380PurposeORFeus expression, WTDepositorInsertORFeus-Hs
UseTagsORF2-3xFlagExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
UseTagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro GFP siRNA
Plasmid#12273DepositorInsertGFP siRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRDA_112
Plasmid#136475PurposeLentiviral expression of AsCas12aDepositorInsertAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
miR34a in pcDNA3.1
Plasmid#78125PurposeMammalian expression of miR34DepositorInsertmiR34 (MIR34A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_118
Plasmid#133459PurposeU6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrRDepositorInsertcontrol guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN-dGBP1-TagBFP
Plasmid#80087PurposeMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection markerDepositorInsertdGBP1-TagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLC-ZsGreen-P2A-Puro
Plasmid#124302PurposeLentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoterDepositorInsertZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDctp-mSox10-Mp1
Plasmid#13513DepositorInsertSox10 (Sox10 Mouse)
UseTagsDct promoter and Mp1-polyAExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV SOCS-3
Plasmid#11486DepositorInsertSOCS-3 (Socs3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC025
Plasmid#227597PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motifDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC026
Plasmid#227598PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
