-
Plasmid#119740PurposeChemogenetics expression plasmidDepositorUseTagsExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
VCP(DK0)-EGFP
Plasmid#23974DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationE305QE578QPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
RfxCas13d-backbone
Plasmid#165078PurposeExpression of RfxCas13d sgRNAs from a U6 promoterDepositorInsertScaffold gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3A
Plasmid#35521PurposeMammalian expression of human DNMT3A with myc tagDepositorInsertDNMT3A (DNMT3A Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
UseTagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialMutationPromoterpDABAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GFP10-ZIP-GFP11
Plasmid#182243PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)DepositorInsertGFP10-ZIP-11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only