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PurposeFor plant prime editing in rice plants
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHUE411
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Vector typePlant Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNls-nCas9-NC-nls-MLV-Nls
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Alt namenCas9-NC-MLV
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Alt nameCas9 nickase
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SpeciesSynthetic
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MutationD10A in SpCas9
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH-ePPE was a gift from Caixia Gao (Addgene plasmid # 183097 ; http://n2t.net/addgene:183097 ; RRID:Addgene_183097) -
For your References section:
An engineered prime editor with enhanced editing efficiency in plants. Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao X, Gao C. Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w. 10.1038/s41587-022-01254-w PubMed 35332341