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Plasmid#101040PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1916A, S1917APromoterCMVAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL
Plasmid#119952PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-1
Plasmid#74985PurposesgKEAP1-1, puromycin selectionDepositorInsertKEAP1 (KEAP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
DHC1 CRISPR
Plasmid#140545PurposeDHC1 tagging CRISPRDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
R-Q111R-N122D
Plasmid#167178PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-R-Q111R-N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged R at 111 to Q and changed D at 122 to NPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ET8-GPIba-high-6His
Plasmid#180251PurposeHigh affinity mutant of human platelet membrane protein GP1b alphaDepositorInserthuman GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)
UseTags6HisExpressionMammalianMutationG233V and M239VPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag5_nostop
Plasmid#153177PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag5
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMG035 MBP-Axin∆BCD-His
Plasmid#154053PurposeExpresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag3_nostop
Plasmid#153175PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag3
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag4_nostop
Plasmid#153176PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag4
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag1_nostop
Plasmid#153173PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag1
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gal4-VP16-HSF1 WT
Plasmid#71726Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RDDepositorInsertGal4 DBD HSF1 VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Human, Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650D
Plasmid#171246PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)DepositorInsert3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Asp (AR-S650D)PromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650A
Plasmid#171245PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)DepositorInsert3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Ala (AR-S650A)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A
Plasmid#171248PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala (AR-W741C-S81A)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81D
Plasmid#171249PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Asp (AR-W741C-S81D)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3F2-mCherry
Plasmid#73418PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.DepositorInsertPromoter 3F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 SARS-CoV-2 S-frag6_nostop
Plasmid#153184PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag6
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221 SARS-CoV-2 S-frag2_nostop
Plasmid#153174PurposeGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS-frag2
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
Plasmid#172195PurposeAAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only