Addgene

Showing: 5701 - 5720 of 6518 results

Results per page:

Sort results by:

pLenti CMV Puro DEST (w118-1) RALB

Plasmid

#107506

Purpose

Expressing RALB, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM4_Deletion_Upstream_gRNA_2

Plasmid

#195135

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 2 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MDM4_Deletion_Upstream_gRNA_1

Plasmid

#195134

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 1 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pINTO-N3::hTET3 V908L

Plasmid

#136370

Purpose

Expresses epitope-tagged human TET3 V908L variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V908L

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pINTO-N3::hTET3 V1089M

Plasmid

#136369

Purpose

Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2x Strep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V1089M

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pINTO-N3::hTET3 A1076T

Plasmid

#136368

Purpose

Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

A1076T

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pINTO-N3::hTET3 R752C

Plasmid

#136367

Purpose

Expresses epitope-tagged human TET3 R752C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

R752C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pINTO-N3::hTET3 F1072C

Plasmid

#136366

Purpose

Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

F1072C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-FLAG-SHLD2

Plasmid

#114117

Purpose

Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCW-eGFP-FHA-SHLD2

Plasmid

#114121

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-S5E2-dTom-nlsdTom

Plasmid

#135630

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV Puro DEST (w118-1) EPB41L1

Plasmid

#107508

Purpose

Expressing EPB41L1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

EPB41L1 (EPB41L1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-S5E2-Gq-P2A-dTomato

Plasmid

#135635

Purpose

AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

Gq-P2A-dTomato

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge