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pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

Plasmid

#113160

Purpose

A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197026

Purpose

Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enOsCas12f1

Use

Tags

Expression

Mammalian

Mutation

D52R / T132R

Promoter

CBh, CMV, hU6

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

Plasmid

#107407

Purpose

Lentiviral expression of gRNA resistant PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

sg resistant PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

sgRNA resistant

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

Plasmid

#113691

Purpose

U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197027

Purpose

Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized enRhCas12f1

Use

Tags

Expression

Mammalian

Mutation

L270R

Promoter

CBh, CMV, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128343

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb2 (Chrnb2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

Plasmid

#159660

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

Plasmid

#159661

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

Plasmid

#159659

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

Plasmid

#109320

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128340

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna5 (Chrna5 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

Plasmid

#160621

Purpose

Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

U6-26:gRNA 4 (pNos): 2.1 aptamer

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

Plasmid

#197024

Purpose

Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

Depositor

Xiangfeng Kong

Article

Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

Insert

humanized OsCas12f1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh, CMV, hU6

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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