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Plasmid#218877PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ChrimsonR-mRuby2-ST
Plasmid#105447PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChrimsonR-mRuby2-ST
UseTagsmRuby2 and soma targeting motifExpressionMammalianMutationK176R in ChrimsonPromoterpCAGGSAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA
Plasmid#163472PurposeDirect-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpAInsertFlpO
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pbabe-cyclinD1+CDK4R24C
Plasmid#11129DepositorUseRetroviralTagsExpressionMammalianMutationCDK4 R24CPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×2-N1
Plasmid#86775PurposeA mammalian expression vector with double EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP-Casp3
Plasmid#81241PurposeA ZipGFP-based Caspase-3 protease reporter T2A mCherryDepositorInsertZipGFP with caspase cleavage sequence T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ISDra2-TnpB-mCherry-U6-ωRNA*
Plasmid#212967PurposeExpression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoterDepositorInsertsISDra2-TnpB-T2A-mCherry
ωRNA*
UseCRISPRTagsNLS and NLS-FLAGExpressionMammalianMutationPromoterCAG, T7 and U6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-d1EGFP
Plasmid#47684PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsd1EGFPExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOS018: ratiometric pHluorin (mitchondrial)
Plasmid#163053Purposeratiometric pHluorin (mitchondrial)DepositorInsertratiometric pHluorin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-lox-hKLF4
Plasmid#20727DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KIT-V5/HIS
Plasmid#201986Purposeexpression of human KIT receptor tyrosine kinase in mammalian cellsDepositorInsertKIT receptor tyrosine kinase (KIT Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-pBAD
Plasmid#108315PurposeGFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.DepositorInsertGFP under control of arabinose-inducible promoter pBAD
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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hCD4-mOrange
Plasmid#110192PurposeEncodes for human CD4 coding sequence tagged with the mOrange FPDepositorInserthCD4 (CD4 Human)
UseTagsmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide
Plasmid#186993PurposeAAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guideDepositorInsertcas7-11-s1006-gggs-d122, non-targeting crRNA guide
UseAAVTagsExpressionMutations1006-gggs-d1221 deletionPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUAL SRBI (GFP)
Plasmid#86979PurposeLentiviral expression construct encoding SR-B1 and GFP from separate promotersDepositorInsertSCARB1 (SCARB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV/hPGKAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only