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Plasmid#114046PurposeExpression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)DepositorInsertMPS1-d60 (TTK Human)
UseTagsExpressionMammalianMutationdelta 60 (deletion of NTE domain)PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d207
Plasmid#114051PurposeExpression of exogenous eGFP HEC1 delta 207DepositorInserteGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6
Plasmid#100140PurposeEncodes amino acids 4421-4834 of HERC2.DepositorInsertHERC2 (HERC2 Human)
UseLentiviralTagsHAExpressionMammalianMutationamino acids 4421-4834PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5
Plasmid#100139PurposeEncodes amino acids 3550-4500 of HERC2.DepositorInsertHERC2 (HERC2 Human)
UseLentiviralTagsHAExpressionMammalianMutationamino acids 3550-4500PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4
Plasmid#100138PurposeEncodes amino acids 2600-3600 of HERC2.DepositorInsertHERC2 (HERC2 Human)
UseLentiviralTagsHAExpressionMammalianMutationamino acids 2600-3600PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2
Plasmid#100136PurposeEncodes amino acids 950-1750 of HERC2.DepositorInsertHERC2 (HERC2 Human)
UseLentiviralTagsHAExpressionMammalianMutationamino acids 950-1750PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1
Plasmid#100135PurposeEncodes amino acids 1-1000 of HERC2.DepositorInsertHERC2 (HERC2 Human)
UseLentiviralTagsHAExpressionMammalianMutationamino acids 1-1000PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCnCas12f1HS_sgRNA_MS13_empty
Plasmid#204999PurposeCnCas12f1 with MS13 sgRNA site for Human cell genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro mCherry-EGFP-LC3B
Plasmid#22418Purposeretroviral expression of mCherry, EGFP-tagged LC3DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (MAP1LC3B Human)
UseRetroviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CTCF-C CRISPR
Plasmid#140647PurposeCTCF tagging CRISPRDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
UseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgAAVS1
Plasmid#85802PurposeCas9/CRISPR vector for cut at human AAVS1 gene intron1DepositorInsertPPP1R12C (PPP1R12C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only