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pMK321 (TOP2A-mAC (Neo))

Plasmid

#140652

Purpose

TOP2A tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-hGH

Plasmid

#182411

Purpose

Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-hGH (GH1 Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74A)

Plasmid

#140731

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-SABR-Backbone

Plasmid

#119051

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MSI2-DCD

Plasmid

#166968

Purpose

Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-DCD

Use

Retroviral

Tags

MSI2-DCD

Expression

Mammalian

Mutation

N495S

Promoter

MSCV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A

Plasmid

#100156

Purpose

Expresses KIF20A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

KIF20A (KIF20A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-KK10-SABR

Plasmid

#119053

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

B2705-KK10-SABR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-STIM1

Plasmid

#89817

Purpose

Express HUMAN STIM1

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

NO TAG

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP11

Plasmid

#65830

Purpose

For membrane contact

Depositor

Kang Shen, Cori Bargmann

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Use

Tags

spGFP11

Expression

Worm

Mutation

Promoter

Prig-3

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Kang Shen, Cori Bargmann

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Use

Tags

spGFP1-10

Expression

Worm

Mutation

Promoter

Prig-3

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E

Plasmid

#100147

Purpose

Expresses EIF3E

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3E (EIF3E Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p7126 pHAGE-P-CMVt-N-HA-GAW-FTH1

Plasmid

#100152

Purpose

Expresses FTH1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shSTIM1

Plasmid

#89816

Purpose

Knockdown human STIM1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 shRNA (STIM1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

Plasmid

#140533

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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nLuc_AP10_PPVs_P9mS

Plasmid

#119302

Purpose

Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p7136 pHAGE-P-CMVt-N-HA-GAW-EIF3H

Plasmid

#100148

Purpose

Expresses EIF3EH

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3H (EIF3H Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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nLuc_AP4_TEVs_P3mS

Plasmid

#119299

Purpose

Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker

Use

Luciferase

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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AP4mS_PPVs_P3_cLuc

Plasmid

#119300

Purpose

Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T12/35A

Plasmid

#47938

Purpose

expresses Myc tagged human MOB1 containing T12A and T35A mutations

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T12A and T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only