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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDest-527

Plasmid

#11518

Purpose

Gateway destination vector for bacterial expression; His tag

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

Plasmid

#66707

Purpose

expresses a single gRNA and VP64-dCas9-VP64

Depositor

Charles Gersbach

Inserts

hU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Expression

Mutation

D10A and H840A

Promoter

hU6 and hUbC

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLY100-RetroEFS-HER2-28BBz-WPRE

Plasmid

#192201

Purpose

Retro-HER2CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
scAAV-Cre

Plasmid

#177933

Purpose

Expresses Cre-recombinase and can be packaged into AAV particles.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Insert

CAG-Cre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CYBL-SPI1-CEBPB-IRF5

Plasmid

#190719

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

SPI1-CEBPB-IRF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pBMN FKBP(DD)-YFP

Plasmid

#31763

Depositor

Thomas Wandless

Article

Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

Insert

FKBP(DD)-YFP-HA (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

F36V, L106P on FKBP12

Promoter

LTR

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pAd5-B1

Plasmid

#122551

Purpose

Block 1 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYC

Plasmid

#141121

Purpose

Lentiviral overexpression of MYC in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Use

Tags

F2A

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pC0046-EF1a-PspCas13b-NES-HIV

Plasmid

#103862

Purpose

Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 Flag Fli1

Plasmid

#66976

Purpose

Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Fli1 (Fli1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2-ChR2-mCherry

Plasmid

#170845

Purpose

AAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Liu et al Elife. 2020 Oct 9;9. pii: 55267. doi: 10

Insert

ChR2-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Dlx

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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