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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Expression

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A Human, (*see below))
humanized birA

Use

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Use

Tags

sfCherry11x4

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Use

Tags

sfCherry11

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
p1A Prk-

Plasmid

#162694

Purpose

p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Prk inactive (K20M S21A native protein, this map …

Promoter

PLtet0-1 promoter

Availability

Academic Institutions and Nonprofits only

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(114) pcDNA3.1-GFP-Flag-JunB-EPEA

Plasmid

#160743

Purpose

Expresses a GFP tagged JunB with Flag and EPEA tags.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

GFP-Flag-JunB-EPEA (JUN Human)

Use

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Mutation

Promoter

T7/CMV

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

Plasmid

#153445

Purpose

E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Use

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG). Argi…

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Mutation

Promoter

LTR-driven expression

Availability

Academic Institutions and Nonprofits only
pEX-PK-hLC3∆G

Plasmid

#61459

Purpose

Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3∆G (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CCR5-VN

Plasmid

#98963

Purpose

FLAG-tagged human CCR5 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

FLAG (dykdddd) epitope tag, Signal/leader sequenc…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Use

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

Plasmid

#111052

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AB (325-557, Δ 368-523)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

Plasmid

#111072

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.5 AB (361-545, Δ395-511)/pET28HMT

Plasmid

#111267

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.5 AB (361-545, Δ395-511)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Use

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pATZ-BFP-HA-GFP11

Plasmid

#177721

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11, HA, and TagBFP

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Availability

Academic Institutions and Nonprofits only

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His-FLAG-Tev_ACTA1 (alpha-actin)

Plasmid

#188452

Purpose

Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTA1 (ACTA1 Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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His-FLAG-Tev_ACTB (beta-actin)

Plasmid

#188453

Purpose

Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTB (ACTB Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge