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pSP64-mHCN2

Plasmid

#53060

Purpose

Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

Insert

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

E55G, R237H, and K283R polymorphisms compared to …

Promoter

SP6

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

Plasmid

#196414

Purpose

Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smFLAG

Use

AAV; Astrocyte-selective

Tags

Lck

Expression

Mammalian

Mutation

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-cMyc T58A

Plasmid

#20075

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

cMyc (MYC Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only
pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NES

Plasmid

#175008

Purpose

Cytosol-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-ARID1A 1759-2285

Plasmid

#39476

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

expresses ARID1A aa1759-2285

Promoter

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
MSCV Puro SCRIB WT

Plasmid

#88886

Purpose

SCRIB WT in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Tags

N/A

Expression

Mutation

N/A

Promoter

MSCV promoter

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-6xgata2aECEbasp

Plasmid

#132968

Purpose

gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

gata2a endothelial enhancer (x6) with a b-actin basal promoter

Use

Gateway vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP V107M

Plasmid

#114180

Purpose

ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP V107M (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

V107M

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1

Plasmid

#64162

Purpose

Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Use

Tags

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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LLP789_αGCN4-DNMT3A-CO

Plasmid

#211783

Purpose

SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Use

Tags

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX10_P2A_Hygro_Barcode

Plasmid

#120479

Purpose

Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC4-RhE-FRB-Fis1

Plasmid

#68056

Purpose

express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

Fis1 (FIS1 Human)

Use

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_mKate2

Plasmid

#96942

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Oleksandr Shcheglovitov, Ricardo Dolmetsch

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

far-red fluorescent protein

Use

Lentiviral

Tags

Expression

Mutation

E87D (please see depositor comments below)

Promoter

CaMKIIa

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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