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pET28a-VHH-ddFLN4-HIS-ybbR

Plasmid

#169137

Purpose

E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

VHH

Use

Tags

6x His tag, ddFLN4 tag, and ybbr tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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actb2-SecAnxaV-mKate2-acry-EGFP

Plasmid

#123628

Purpose

Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

AnnexinA5

Use

Tags

mKate2

Expression

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR-PMG (pXK-1650)

Plasmid

#208858

Purpose

HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Article

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eDrIR1

Plasmid

#183998

Purpose

Optically controlled eDrIR1 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrIR1 (INSR Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

Plasmid

#170488

Purpose

SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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PymeACR1

Plasmid

#153031

Purpose

Prasinophyte anion-conducting channelrhodopsin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

PymeACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-2376

Plasmid

#138974

Purpose

Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI

Use

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TAC-WTSI

Plasmid

#80486

Purpose

piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

WTSI

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-Climp63

Plasmid

#136293

Purpose

expression of Climp63

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Cytoskeletal-linking protein 63 (Ckap4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Ch-gelsolN

Plasmid

#37262

Depositor

Ikuo Wada

Article

Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

Insert

gelsolin (GSN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deleted amino acids 380-731

Promoter

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Use

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Mutation

Promoter

myo-3 and unc-119

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Article

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDN34-BiNLS-hYAP1-1delta

Plasmid

#169471

Purpose

expresses optoYAP in mammalian cells

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

YAP1-1delta (YAP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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