pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA
(Plasmid
#184632)
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PurposeExpresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
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Backbone manufacturerAddgene #20297
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGad67
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SpeciesM. musculus (mouse)
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GenBank IDNM_008077.5
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Entrez GeneGad1 (a.k.a. EP10, Gad-1)
- Promoter EF1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Ascl (not destroyed)
- 3′ cloning site Nhel (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.26.470142v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA was a gift from Nicolas Tritsch (Addgene plasmid # 184632 ; http://n2t.net/addgene:184632 ; RRID:Addgene_184632) -
For your References section:
Inhibitory co-transmission from midbrain dopamine neurons relies on presynaptic GABA uptake. Melani R, Tritsch NX. Cell Rep. 2022 Apr 19;39(3):110716. doi: 10.1016/j.celrep.2022.110716. 10.1016/j.celrep.2022.110716 PubMed 35443174
