Addgene

We narrowed to 14,767 results for: grna

Showing: 5581 - 5600 of 14767 results

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pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220119

Purpose

Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1

Plasmid

#220120

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA6

Plasmid

#220122

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220124

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220127

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

HA tag

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

Plasmid

#226921

Purpose

Encoding SauriABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold

Plasmid

#226933

Purpose

Encoding Nme2ABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nNme2Cas9

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

Plasmid

#203448

Purpose

Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

Plasmid

#203452

Purpose

Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Olactb and U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-DIO-NLS-mRuby3(with gRNA scaffold)

Plasmid

#199583

Purpose

Expresses NLS-mRuby3 in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

NLS-mRuby3

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1a

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse cGAS #1-gRNA-Cas9-Puro

Plasmid

#186890

Purpose

gRNA targeting mouse cGAS (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Cgas Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse cGAS #2-gRNA-Cas9-Puro

Plasmid

#186891

Purpose

gRNA targeting mouse cGAS (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Cgas Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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