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Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-sfGFP
Plasmid#61058PurposeA direct fusion of sfGFP to the 24xGCN4_v4 peptide arrayDepositorInsert24xGCN4_v4 peptide array and sfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
UseTagsLyn11 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT PINK1 N-myc
Plasmid#13313DepositorInsertPINK1 (PINK1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-EGFP
Plasmid#153163PurposeMouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
RA-Sec61β
Plasmid#153978PurposeDimerization dependent fluorescent protein RA domain fused to ER protein Sec61βDepositorInsertSec61 Transolocon Subunit Beta (SEC61B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2-Flag
Plasmid#180407Purposelentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) geneDepositorInsertTGM2 (TGM2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDsRed2-Keap1
Plasmid#21551DepositorInsertkelch-like ECH-associated protein 1 (KEAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon4000
Plasmid#126208Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon4000
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b-V106W
Plasmid#215825PurposeExpress CBE6b V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6b V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VARP
Plasmid#42312DepositorInsertVARP (ANKRD27 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry
Plasmid#193465PurposeDn29 Donor with promoter for mcherry and puro expressionDepositorInsertDn29_attP
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-N2c_envA-IRES-Neo
Plasmid#172368PurposeGeneration of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)DepositorInsertenvA + Neo
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN1-fDIO-tTA
Plasmid#166597PurposeEncodes Flp-dependent tTADepositorInserttTA
UseAAVTagsExpressionMutationPromoterhuman synapsin 1Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro
Plasmid#171991PurposeDelivers all prime editing nickase components in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only