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Plasmid#157889PurposeExpresses the DRT type 3 defense systemDepositorInsertDRT type 3 (RT + RT)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-RC3AI
Plasmid#78908PurposeIndicator of caspase-3-like protease activityDepositorInsertRC3AI
UseLentiviralTagsHAExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL055 pDESTattB_cryaa:Venus
Plasmid#213509PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCE048-SiT-Cas12a
Plasmid#128124PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-minCMV-mCherry
Plasmid#27970DepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-42)
Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_WT MDA5
Plasmid#216798PurposeExpresses wild type (wt) melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…ExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQXCINeo-LOXL2-BC
Plasmid#134763Purposea retroviral vector to express lysyl oxidaseL2DepositorInsertLOXL2 (LOXL2 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCB1_STOP
Plasmid#221424PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCB1 (ABCB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR4-YFP
Plasmid#13018DepositorInsertToll-Like Receptor 4 (TLR4 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUltra-hot
Plasmid#24130Purpose3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interestDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only