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Plasmid#53054PurposeExpresses alpha subunit of F656A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Phenylalanine 656 to AlaninePromoterSP6Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a Y652A
Plasmid#53053PurposeExpresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Tyrosine 652 to AlaninePromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-bPAC(R278A)-Myc-eYFP
Plasmid#165488PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertbPAC(R278A)-Myc-eYFP
UseCdna expression, xenopus oocyteTagsEYFP and MycExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S620T
Plasmid#53059PurposeExpresses alpha subunit of S620T hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Serine 620 to ThreoninePromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-Glyco-Venus-bPAC(S27A)
Plasmid#165490PurposeXenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activityDepositorInsertGlyco-Venus-bPAC(S27A)-Myc
UseCdna expression, xenopus oocyteTagsMyc and VenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hKCNQ1
Plasmid#53048PurposeExpresses alpha subunit for hKCNQ1 channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT1
Plasmid#73943PurposeXenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tagDepositorInsertPepT1 (SLC15A1 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ mWnt5a
Plasmid#102860PurposeExpresses mouse Wnt5a in mammalian cells, zebrafish or XenopusDepositorInsertMouse Wnt5a
UseZebrafish expression; xenopus expressionTagsExpressionMammalianMutationPromoterCMV; SP6Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165489PurposeXenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseCdna expression, xenopus oocyteTagsVenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCav1
Plasmid#194290PurposeMultisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRTDepositorInsertcaveolin1 (cav1.S Frog)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM 3B-His
Plasmid#172192PurposeCoding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned mediumDepositorInserthFAM 3B
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCMV:ECFP(loxP)(FRT)HNF1B
Plasmid#31439DepositorInsertHNF1B (HNF1B Human)
UseCre/Lox; Flp/frtTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del
Plasmid#30528DepositorInsertHNF1B (HNF1B Human)
UseCre/Lox; Flp/frtTagsMycExpressionMammalianMutationP328L329delCCTCTPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins
Plasmid#31440DepositorInsertHNF1B (HNF1B Human)
UseCre/Lox; Flp/frtTagsMycExpressionMammalianMutationA263insGGPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 162-267
Plasmid#86002Purposebacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53DepositorTypeEmpty backboneUseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCav1 in pT3TS-Dest
Plasmid#194293PurposeIn vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZWDepositorInsertcaveolin (cav1.S Frog)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM3B-HA
Plasmid#180145PurposeCoding sequence of human FAM3B pCS2 with C terminal HA tagDepositorInsertFAM3B (FAM3B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUG36-NES-EGFP-cPHx3
Plasmid#183668PurposePtdIns(3,4)P2 biosensor for expression in yeastDepositorInsertPLEKHA1 (PLEKHA1 Frog, Human)
UseTagsX. laevis map2k1.L(32-44)-EGFPExpressionYeastMutationAmino acids 169-329 (tandem trimer)PromoterMET25Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUG34-NES-EGFP-cPHx3
Plasmid#183669PurposePtdIns(3,4)P2 biosensor for expression in yeastDepositorInsertPLEKHA1 (PLEKHA1 Frog, Human)
UseTagsX. laevis map2k1.L(32-44)-EGFPExpressionYeastMutationAmino acids 169-329 (tandem trimer)PromoterMET25Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only