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pCX138

Plasmid

#229213

Purpose

CRISPR array plasmid for NOTCH2 (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol (2025)

Insert

CRISPR array plasmid for NOTCH2 (Spacers 25-48)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mCherry-EGFP-LC3B

Plasmid

#170446

Purpose

Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

mCherry-GFP-LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro

Plasmid

#8453

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro

Plasmid

#86708

Purpose

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

Depositor

Christoph Bock

Article

Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

Plasmid

#172910

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Voltron2-ST

Use

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV2/9n

Plasmid

#112865

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap9

Use

AAV

Tags

Expression

Mutation

Promoter

Truncated P5

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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SB100X in pCAG globin pA

Plasmid

#127909

Purpose

Sleeping Beauty Transposase expression vector driven by the CAG promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

Sleeping Beauty Transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hTERT-IRES-hygro

Plasmid

#85140

Purpose

Lentiviral expression of hTERT, used to create immortalized cell lines

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G13D

Plasmid

#82808

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G13D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-NanoLuc

Plasmid

#188116

Purpose

Template plasmid to transcribe circular RNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-ARID1A

Plasmid

#39311

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mTagBFP2

Plasmid

#113725

Purpose

Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

minimal TATA-box promoter with low basal activity

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PE-bacteria

Plasmid

#172715

Purpose

Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

Cas9n-linker-M-MLV2

Use

Tags

Expression

Bacterial

Mutation

H840A of Cas9

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Use

Tags

Expression

Bacterial

Mutation

E32K

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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