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Plasmid#205248PurposeIn vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.DepositorInsertKRAB-dSpCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9A-HA
Plasmid#209213PurposeMammalian expression of ATP9ADepositorInsertATP9A (ATP9A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VCre
Plasmid#89575PurposeExpresses VCre under pCAGDepositorInsertVCre recombinase
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mRhubarb720
Plasmid#197212PurposeExpresses the protein of mRhubarb720 in mammalian cellsDepositorInsertmRhubarb720
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6011nes
Plasmid#108652PurposeAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-ERK-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAD16_CAD_12-490_pvp008
Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAf
Plasmid#54323PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-GFP
Plasmid#13772PurposeFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertEGFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-DsRed
Plasmid#13771PurposeFlp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertDsRed2
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX165-eSpCas9
Plasmid#140564PurposeExpresses eSpCas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationK848A K1003A R1060APromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ROSAr
Plasmid#54324PurposeTALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmidDepositorInsertTALEN for the murine ROSA26 locus
UseMouse Targeting and TALENTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2623 (pCas6_Vch)
Plasmid#200895PurposeExpresses human codon-optimized V. cholerae Cas6 from a CMV promoter.DepositorInsertVchCas6
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
P628_SpCas9-RecA
Plasmid#87263PurposeHuman codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiencyDepositorInsertRecA
UseCRISPRTagsFLAGExpressionMammalianMutationnoPromoterCBhAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEc
Plasmid#193834PurposeExpress TadCBEc (with SpCas9) in mammalian cellsDepositorInsertTadA-CDc-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-mSA*
Plasmid#182952PurposeExpress SpCas9-mSA*DepositorInsertSpCas9-mSA*
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_TagRFP
Plasmid#161934PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG:myr-mRFP1
Plasmid#32604DepositorInsertmyr-mRFP1
UseMouse TargetingTagsExpressionMutationPromoterpCAGGsAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-hCas9D10A
Plasmid#52101PurposeThe expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoterDepositorInserthCas9D10A
UseTagsSV40 NLSExpressionMammalianMutationD10APromoterCAGAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only