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Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-Q61L
Plasmid#12981DepositorInsertRac1 constitutively active (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorInsertRhoA DN (RHOA Human)
UseTagsEGFPExpressionMammalianMutationT19N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-Q63L
Plasmid#12968DepositorInsertRhoA constitutively active (RHOA Human)
UseTagsEGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE
Plasmid#89608PurposeExpression of Luciferase in human cells. 3rd generation lentiviral vectorDepositorInsertLuciferase
UseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-IC3-FLAG-Tev-ZZ
Plasmid#170311PurposePlasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.DepositorInsertOuter Dynein Arm Intermediate Chain 3
UseTagsFLAG, ZZExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS
Plasmid#90996PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAIP
Plasmid#74171PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorInsertsUseTagsGFPExpressionWormMutationPromoterhis-72 and unc-119Available sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
L1
Plasmid#21200DepositorUseRetroviralTagsExpressionMutationMek2 K101A fused to ER*, catalytically inactivePromoterAvailable sinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7746_v3
Plasmid#126856Purpose7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffoldsDepositorInsertII_CS3_7746_v3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only