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Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV:AsCpf1-2A-GFP-U6-AAVS1-sg
Plasmid#194716PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locusDepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 iFE
Plasmid#190138PurposeExpresses SpCas9-POLB (iFE)DepositorInsertSpCas9-POLB (HiFi iFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ttHsCas12a
Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyTagsExpressionMutationD156RPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorInsertNAP1L1 (NAP1L1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-3Myc:HaloTag:3Myc
Plasmid#181943PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertHaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDC2
Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-spGFP(11)
Plasmid#190257PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertspGFP(11)
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mScarlet
Plasmid#179818PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mNG
Plasmid#179821PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VPS15-TSF
Plasmid#203563PurposeMammalian expression of VPS15 with TwinStrep-Flag tagDepositorInsertVPS15 (PIK3R4 Human)
UseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NASP-Halo
Plasmid#175340PurposeProduction of HaloTag fusion proteinDepositorInsertNASP (NASP Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-CLIPtag
Plasmid#179787PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertCLIP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-puro-3Ty:tagRFPt:3Ty
Plasmid#201089PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserttagRFPt
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mCherry
Plasmid#179789PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-3mNG
Plasmid#179832PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3x mNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-mScarletI
Plasmid#179820PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mCardinal
Plasmid#179796PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCardinal
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only